Experimental Biophotonics focuses on advanced light microscopy techniques and methodologies applied to the study of nano- and microstructures characteristics and interactions with living cells including assessments of possible cytotoxicity and cancer cell diagnostics.


Advanced light microscopy – innovative technologies

  • theoretical study of imaging processes in advanced, especially holographic and confocal light microscopy, simulation and experimental verification of results
  • investigation of coherence effects, optical and coherence vortices in holographic imaging, imaging in turbid media by coherence gating
  • development of coherence controlled and SLM optical systems for holographic and multimodal (correlative) microscopy
  • development of advanced image-processing methods for holographic reconstruction, quantitative phase evaluation, dynamic phase differences, pattern and process recognition

Evaluation of live cell behaviour & analysis of micro- and nanostructures

  • biophysics of quantitative evaluation of live cell behaviour developed with coherence-controlled holographic microscopy (CCHM)
  • subtoxic impact/toxicity of nanomaterials assessment by measurement of intracellular dry mass translocation with CCHM
  • in vitro qualification of cancer cell malignancy with CCHM classification of cell behaviour patterns
  • observation and validation of nanostructure properties and their interactions with living cells with advanced light microscopy



Published paper "Quantitative phase imaging unravels new insight…

New paper "Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell… Read more »


​​2nd international meeting - PHASE IN BRNO 2018: Dry-Mass…

Date: 22-24 October, 2018 Venue: CEITEC BUT, Purkynova 656/123, Brno, Czech Republic The meeting will… Read more »


Published paper "Dual-polarization interference microscopy for…

New paper "Dual-polarization interference microscopy for advanced quantification of phase associated with the image… Read more »


Published paper "Distinctive behaviour of live biopsy-derived…

New paper "Distinctive behaviour of live biopsy-derived carcinoma cells unveiled using coherence-controlled holographic… Read more »